The present study was conducted to record the prevalence of Bovine Tropical Theileriosis (BTT) caused by the protozoan parasite, Theileria annulata in cattle. Animals were screened on the basis of clinical signs like fever, anorexia, with or without superfi cial lymph node enlargement, blood smear and lymph node biopsy examination for presence of Theileria annulata. Out of 150 cattle screened, 35 (23.33%) were found to be positive for Tropical theileriosis. The prevalence of Tropical theileriosis was higher in female (25.45%) than male (17.5%). The prevalence of Tropical theileriosis in cattle in respect to age showed highest prevalence (24.34%) in adult cattle of above 3 years age, followed by 23.80 % in the age group of 1 to 3 years and 14.28% in 0 to 1 year. The prevalence of Tropical theileriosis in respect to breed was maximum in HF cross (29.85 %) followed by 23.33 % in Jersey cross, 15.38 % in Sahiwal, and 14.81% in Gir.